Universal Genome Engineering (HiUGE)

“HiUGE” is the acronym Duke University researchers have developed with which they are able to fluorescently tag antibodies using gene editing technology. “HiUGE uses CRISPR to insert a short genetic sequence into the target protein’s gene via an adenovirus delivery system. This molecular tag which codes for a segment of amino acid sequences is detected by a highly sensitive antibody which is significantly more reliable than many of the commercially-available antibodies for research on the market. Different combinations of these CRISPR tags have the potential to mark hundreds of proteins and the technology can easily be adapted to automated, high-throughput laboratory imaging processes.” MORE

Image Credit:   Duke University