iPALM Microscopy
February 10, 2009 | Terry Sharrer
Harald Hess and colleagues at the Howard Hughes Medical Institute’s research facilities in Loudoun County, VA have reported (in PNAS) their development of a super resolution technique under visible light-called interferometric photoactivated localization microscopy (iPALM). By detecting fluorescent light from both above and below a labeled molecule, and then splitting the beams for three separate cameras, they created a 3D image of a protein in a space of about 20 nanometers. The next step is to see such targets in living cells. MORE
See images of cell structures created using iPALM Technology Review and Howard Hughes Medical Institute