Instability in Growing Organoids Inside Extracellular Matrix Hydrogel
December 8, 2020 | Terry Sharrer
University of Texas at Austin researchers have found irregularities in growing organoids in hydrogel. “The location-dependent heterogeneity of organoids in a hydrogel dome substantially perturbed the transcriptome profile associated with epithelial functions, cytodifferentiation including mucin 2 expression, and morphological characteristics. This heterogeneous phenotype was significantly mitigated when the Wnt3a was frequently replenished in the culture medium. Our finding suggests that the morphological, transcriptional, translational, and functional heterogeneity in conventional organoid cultures may lead to a false interpretation of the experimental results in organoid-based studies. MORE
Image Credit: University of Texas at Austin and Cell.com