Pytheas- a software package for the automated analysis of RNA sequences

“To provide an analytical tool for sequence characterization of modified RNAs, we [computational biologists at the Scripps Research Institute] developed Pytheas, an open-source software package for automated analysis of tandem MS data for RNA. The main features of Pytheas are flexible handling of isotope labeling and RNA modifications, with false discovery rate statistical validation based on sequence decoys. We demonstrate bottom-up mass spectrometry characterization of diverse RNA sequences, with broad applications in the biology of stable RNAs, and quality control of RNA therapeutics and mRNA vaccines.”   MORE

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