Plant-Derived Proinsulin
August 29, 2023 | Terry Sharrer
“Diabetes Mellitus is a silent epidemic . . . Therefore, we [at the University of Pennsylvania] engineered proinsulin in plant cells to facilitate oral delivery. Stability of the proinsulin gene and expression in subsequent generations, after removal of the antibiotic-resistance gene, was confirmed using PCR, Southern and western blots. Proinsulin expression was high (up to 12 mg/g DW or 47.5% of total leaf protein), stable up to one year after storage of freeze-dried plant cells at ambient temperature and met FDA regulatory requirements of uniformity, moisture content and bioburden. MORE
Image Credit: ScienceDirect