Precision and accuracy of receptor quantification surfaces using DNA-PAINT

“DNA-PAINT (Point Accumulation for Imaging in Nanoscale Topography) has become one of the quantitative methods for single-molecule localization microscopy (SMLM). It is based on the short transient interaction of a labeled DNA probe (imager) to a complementary DNA target molecule (docking). The bound time of the DNA–DNA interaction is highly dependent on the length DNA duplex that is formed and can be optimized by tuning the length base pair (bp) leading to typical bound times in the order of milliseconds or seconds and independent of the imager concentration.”    MORE

Image Credit:   Pubs.acs.org