Mesoscopic oblique plane microscopy

“By augmenting the illumination angle of the light sheet with a transmission grating, we [researchers at Johns Hopkins University] improved the axial resolution approximately sixfold over existing methods and approximately twofold beyond the diffraction limitation of the primary objective lens. We demonstrated a FOV up to 5.4mm×3.3mm with resolution of 2.5µm×3µm×6µm, allowing volumetric imaging of 3D cellular structures with a single scan. Applying Meso-OPM for in vivo imaging of zebrafish larvae, we report here in toto whole-body volumetric recordings of neuronal activity at 2 Hz volume rate and whole-body volumetric recordings of blood flow dynamics at 5 Hz with 3D cellular resolution.”       MORE

Image Credit: Optica Publishing Group