New CRISPR gene-editing system can “drag-and-drop” DNA in bulk

A new technique has been added to the CRISPR gene-editing toolbox. Known as PASTE, the system uses virus enzymes to “drag-and-drop” large sections of DNA into a genome, which could help treat a range of genetic diseases.  . . . .  In a series of tests the [MIT] team put the PASTE system to work in human liver cells, T cells and lymphoblasts, inserting 13 different genes into nine locations in the genome. The success rate was up to 60%, and generated very few errors at the site of insertion. Tests in mice with “humanized” livers only worked in about 2.5% of cells, however.”     MORE

Image Credit:  NewAtlas