How Single Molecule Taq Polymerase Works
May 24, 2022 | Terry Sharrer
Biochemists at University of California-Irvine report “single-walled carbon nanotube transistors recorded the motions of Taq molecules processing matched or mismatched template–deoxynucleotide triphosphate pairs from 22° to 85°C. By using four enzyme orientations, the whole-enzyme closures of nucleotide incorporations were distinguished from more rapid, 20-μs closures of Taq’s fingers domain testing complementarity and orientation.” MORE
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