Single Cell Electroporation
May 9, 2017 | Terry Sharrer
A lab team at Rutgers has combined a microfluidics device with electroporation apparatus to open cells as they flow through channels, allowing them to absorb nucleic acids with little damage to the membrane. “The novelty of this report is the impedance detection of membrane permeabilization in a continuous flow environment at unprecedented sensitivity, an accomplishment not previously reported in literature. By monitoring changes in the electrical characteristics of individual cells when exposed to short, high-strength electric fields, the team was able to identify when a cell has become permeabilized and determine the conditions which led to molecular delivery while preserving cell viability.” MORE