Gene Editing for DNA Repair

Enhanced prime editing systems
“Toward the goal of enabling a wide range of sequence changes, we [a collaboration of Broad Institute, Princeton, UCSF, et al] developed prime editing, a versatile gene editing approach that can install all types of targeted DNA base pair substitutions, small insertions, small deletions, and combinations thereof without requiring double-strand DNA breaks (DSBs) or donor DNA templates. Prime editing has been broadly applied to introduce genetic changes in flies, rice and wheat, zebrafish, mouse embryos, post-natal mice, human stem cells, and patient-derived organoids. . . To maximize the utility of prime editing, we sought to identify cellular determinants of prime editing outcomes and use the resulting insights to develop improved prime editing systems.” MORE
Image Credit: Cell.com