Spatial Transcriptional Mapping of the Human Nephrogenic Program

“To establish a framework for human nephrogenesis, we (computational biologists at the University of Southern California, Keck School of Medicine, and collaborators) spatially resolved a stereotypical process by which equipotent nephron progenitors generate a nephron anlagen, then applied data-driven approaches to construct three-dimensional protein maps on anatomical models of the nephrogenic program. Single cell RNA sequencing identified novel progenitor states which were spatially mapped to the nephron anatomy enabling the generation of functional gene-networks predicting interactions within and between nephron cell-types. Network mining identified known developmental disease genes and predicts new targets of interest.”   MORE

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