Reaching Greater PCR Speeds, Specificity, and Fidelity

“KOD is a recombinant DNA polymerase based on the endogenous KOD1 polymerase found in Thermococcus kodakaraensis. Because it has 3’ to 5’ exonuclease activity, KOD is able to detect and remove incorrect base pairs during DNA synthesis. This makes it a high-fidelity polymerase. MilliporeSigma now offers a new KOD OneTM polymerase, featuring UKOD, a genetically modified hot-start polymerase that offers even higher fidelity. In fact, when evaluating the number of misincorporated bases per 100,000 bases, the KOD OneTM polymerase shows approximately 80-fold higher fidelity than Taq.”   MORE

Image Credit:  The-scientist.com