CRISPR-LIGHT tool for genetic screening tool for organoids

CRIPSR-Lineage Tracing at Cellular resolution in Heterogeneous Tissue (CRISPR-LICHT) is the term researchers at the Institute of Molecular Biology of the Austrian Academy of Sciences has used to expand the size of brain organoids.  “The key trick was to apply a guide RNA, but also a genetic barcode, a piece of DNA that we add to the genome of the cells we use to grow organoids. This allows us to see the entire cell lineage of each organoid, while a second barcode lets us to count the number of cells generated by each starting cell. This reduces noise and so we can determine the effect of each guide RNA on the number of cells produced during organoid growth.”  MORE

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