Regenerative Medicine’s Gene Editing Toolbox
Scientists at the Wake Forest Institute for Regenerative Medicine have figured out how to control the off-targeting problem of CRISPR-Cas 9 gene editing. Their solution was to incorporate lentivirus-like bionanoparticles into the Cas 9 messenger RNA. “By combining the transient expression feature of nanoparticle-delivery strategies while retaining the transduction efficiency of lentiviral vectors, we have created a system that may be used for packaging various editor protein mRNA for genome editing in a ‘hit and run’ manner, . . . . This system will not only improve safety but also avoid possible immune response to the editor proteins, which could improve in vivo gene editing efficiency which will be useful in research and clinical applications.” MORE
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