Using CRISPR/Cas for “Knock In” Cassettes
June 30, 2015 | Terry Sharrer
“Knock out” mice—so called because one of several means has been used to inactivate a murine gene—have been invaluable research animals for studying how genes work. The CRISPR/Cas system is capable of introducing a “cassette” of DNA that conveys a new characteristic (e.g. expression of green fluorescent protein in neurons) resulting in “knock in” mice. The processes for inactivating genes are highly inefficient, perhaps no more than 5%, while this piece describing research at Tokyo Medical and Dental University has achieved up to 50% transformation rate. MORE
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